Salt gradient chromatographic separation of chondroitin sulfate disaccharides
نویسندگان
چکیده
منابع مشابه
Divergent Synthesis of Chondroitin Sulfate Disaccharides and Identification of Sulfate Motifs that Inhibit Triple Negative Breast Cancer
Glycosaminoglycans (GAGs) regulate many important physiological processes. A pertinent issue to address is whether GAGs encode important functional information via introduction of position specific sulfate groups in the GAG structure. However, procurement of pure, homogenous GAG motifs to probe the "sulfation code" is a challenging task due to isolation difficulty and structural complexity. To ...
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While some complementary and alternative techniques have been studied scientifically, high-quality data regarding safety, effectiveness, and mechanism of action are limited or controversial for most therapies. Whenever possible, it is recommended that practitioners be licensed by a recognized professional organization that adheres to clearly published standards. In addition, before starting a n...
متن کاملBiosynthesis of Chondroitin Sulfate
A microsomal preparation from chick embryo cartilage has been shown to catalyze the incorporation of sulfate-36S from 3’-phosphoadenosine 5’-phosphosulfate-3% into microsomal glycosaminoglycan. This enzyme preparation is the same preparation that previously has been shown to catalyze the formation of chondroitin (nonsulfated) from sugar nucleotide precursors. The microsomal sulfate acceptor app...
متن کاملBiosynthesis of Chondroitin Sulfate
The role of phospholipids in the activity of UDP-D-galactose:D-xylose galactosyltransferase (galactosyltransferase I) from embryonic chick cartilage was investigated. Phospholipase C treatment of particulate galactosyltransferase I caused inactivation of this enzyme to the extent of 60 to 70% as well as hydrolysis of 75 to 80% of the membrane phospholipids. Addition of phospholipid restored act...
متن کاملHigh performance liquid chromatographic assay of disaccharides and oligosaccharides produced by the digestion of glycosaminoglycans with chondroitin sulphate lyases.
In high performance liquid chromatographic procedures hitherto described, SiO2, NH2 and RP columns have been used for the analysis of disaccharides produced by the digestion of glycosaminoglycans with the chondroitin sulphate lyases AC and ABC. The use of a potent anion exchanger offers the following advantages over these columns: superior separation characteristics for non-sulphated disacchari...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2020
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2020.460979